Figure 4 | Scientific Reports

Figure 4

From: Point mutations in Candida glabrata 3-hydroxy-3-methylglutaryl-coenzyme A reductase (CgHMGR) decrease enzymatic activity and substrate/inhibitor affinity

Figure 4

Graphic representation, overexpression, and detection of the recombinant CgHMGRE680Q, CgHMGRE711Q, CgHMGR805A, CgHMGRM807R and CgHMGRE680Q-M807R proteins. (a) Cartoon illustration of the p-MBP-CgHMGR (where MBP refers to the maltose-binding protein), which was used to obtain and express mutant CgHMGR proteins. (b) SDS-PAGE of the expression of the mutated proteins: Lane 1, molecular weight marker; lanes 2, 4, 6, 8 and 10, extract of cells before induction; lanes 3, 5, 7, 9 and 11, extract of cells after induction with IPTG. The full-length gel is included in Supplementary Information (Fig. S4a). (c) Example of the verification of protein identity by Western blot based on anti-MBP antibodies: Lane 1, molecular weight marker; lane 2, uninduced cell extract; lane 3, induced cell extract. The full-length blot is also shown in Supplementary Information (Fig. S4b).

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