Figure 4 | Scientific Reports

Figure 4

From: A new, fluorescence-based method for visualizing the pseudopupil and assessing optical acuity in the dark compound eyes of honeybees and other insects

Figure 4

The dimensions of the induced fluorescent pseudopupil depends on the numerical aperture (NA) of the objective lens. (a) Heatmap of the induced fluorescent pseudopupil in a honeybee forager eye stained with Lucifer Yellow (see “Methods”) and acquired with a Nikon SMZ18 microscope fitted with a P2-EFLGFP-B filter cube, a 1 × SHR PlanApo objective with two different numerical apertures (NA), 0.15 (top) and 0.075 (bottom). (b) Relative pixel values of the rectangular area in (a) showing that the two pseudopupils have the same centre despite subtending a broader area when the NA is higher (orange line).

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