Figure 5 | Scientific Reports

Figure 5

From: Riluzole-induced apoptosis in osteosarcoma is mediated through Yes-associated protein upon phosphorylation by c-Abl Kinase

Figure 5

Riluzole induces phosphorylation of YAP at Y357 to promote binding of YAP with p73. (A) Whole cell extracts of treated LM7 cells with DMSO, Riluzole (50 μM) or Riluzole (50 μM) + PPY A (20 nM) were blotted with rabbit anti-YAP (phosphoY357) antibody and the blot was reprobed with mouse anti-YAP antibody. (B) Co-immunoprecipitation was performed to assess interaction between endogenous YAP and p73 using whole cell extracts. Samples were immunoprecipitated with mouse anti-YAP antibody and the western blot was probed with rabbit anti-p73 antibody, the blot was reprobed with mouse anti-YAP antibody. An asterisk next to the band indicates a nonspecific band. (C) The densities of the immunoprecipitated p73 and YAP were quantified to measure the ratio of p73/YAP. These experiments were repeated three times to confirm the results, p value < 0.05**, IP immunoprecipitation.

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