Figure 1 | Scientific Reports

Figure 1

From: Insights into phenotypic differences between humans and mice with p.T721M and other C-terminal variants of the SLC26A4 gene

Figure 1

Generation of mice with Slc26a4 p.H721M variant using CRISPR/Cas9. (A) Design diagram. SgRNA for CRISPR/Cas9 and silent mutations for enzyme cutting sites as a check were designed to incorporate the c.2162C > T (p.H721M) variant into the genome of C57BL/6 mice. To generate the Slc26a4 p.T721M variant, the codon “ACG” was mutated to “ATG”. (B) Sanger sequencing was performed to confirm the nucleotide change in transgenic mice. The sequence was read in reverse.

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