Figure 6
From: Modulation of circadian clock by crude drug extracts used in Japanese Kampo medicine
Involvement of AKT signaling in the circadian rhythm. (A) Schematic of interactions of the AKT pathway. (B,C) Effects of AKT activator SC79 (B) and AKT inhibitor A-443654 (C) at different concentration. Luminescent traces (left) and dose-dependent effects on period (middle) and phase (right) in U2OS cells. (D) Effects of the knockdown of AKT1/2/3 by siRNA. U2OS cells were transfected with negative control (NC) siRNA, AKT1 siRNA, AKT2 siRNA, or AKT3 siRNA. Luminescent traces (left) and effects on circadian period (middle) in U2OS cells. Efficiency of knockdown of AKT was confirmed using RT-qPCR (right). Values are the averages of six replicates ± SD and were analyzed using one way ANOVA, followed by Dunnett’s multiple comparisons test (*p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001).
