Figure 3

(A–D). AA amyloid from cattle cross seeds human Aβ42 in vitro. (A) 3 µM recombinant Aβ42 and AA amyloid isolated from a bovine kidney (400 × dilution from the stock as final concentration) were monitored separately in the presence of ThT at 37 °C. Aggregation traces of different concentrations of Aβ42, i.e., 1.6 µM (B), 3 µM (C) and 5 µM (D) with and without bovine AA amyloid (400 × dilution from the stock as final concentration) at 37 °C. The data is presented as mean with error bars (SD from four replicates). (E,F) AA amyloid fibrils from cattle cross seed human Aβ40 in vitro. Aggregation traces of different concentrations of Aβ40, i.e., 5 µM (E) and 10 µM (F) with and without AA amyloid (400 × dilution from the stock as final concentration) at 37 °C. The data is presented as mean with error bars (SD from four replicates).