Figure 1 | Scientific Reports

Figure 1

From: Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18

Figure 1

The two cell lines MBU-7 and MBU-8 differ in trisomies 8 and 18 and a partial trisomy of 7. (a) Representative karyotype images of MBU-7 and MBU-8 showing cytogenetic differences: trisomy of chromosomes 7 and 18, and tetrasomy 8p. (b) Distribution of copy number variations in chromosomes 7, 8 and 18 in MBU-7 and MBU-8 as determined by CytoScan 750 k Array analysis. Blue, gain; red, loss; purple, region of heterozygosity. (c) FISH with probes for EZH2 at 7q36 (green), the centromeric region at 7p11.1-q11.1 (blue) and KMT2E at 7q22 (red). (d) FISH with probes for IGH at 14q32.3 (green), the centromeric region at 8p11.1-q11.1 (blue) or MYC at 8q24 (red). (e) FISH with probes for BCL2 at 18q21 (orange). Arrows indicate loci that differ between MBU-7 and MBU-8. (f) Quantification of the FISH signal represented in (c–e) by the Metafer platform in MBU-7 and MBU-8 after 2–4 passages (p2-4) and 18–20 passages (p18-20).

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