Figure 2
From: Optimizing the evaluation of gene-targeted panels for tumor mutational burden estimation
TMB estimation results for gene panels in different datasets. (A, B) Linear fit with 95% confidence intervals of panel-based TMB estimated by the F1CDx and the MSK against WES-based TMB in the hypermutation-included dataset. (C, D) Linear fit with 95% confidence intervals of panel-based TMB estimated by the F1CDx and the MSK against WES-based TMB in the non-hypermutation dataset. The R-squared values are lower in the non-hypermutation dataset than in the hypermutation-included dataset for both panels. (E, F) The correlation between the estimated bias and TMB size (Pearson correlation: F1CDx: − 0.1066, P value: 3.2 × 10–27; MSK: − 0.1047, P value: 2.4 × 10–26). The X axis is the WES-based TMB of the patient and the Y axis is calculated as the panel-based TMB divided by the WES-based TMB. A log-2 scale is used for the Y axis of the graph. Patients with TMB > 500 are not shown.
