Figure 2

(A) Example images of changing LDH staining distribution of burns of older age compared against matched control skin. Demographics of patient, injury type, time since injury and body part section taaken from presented in table. Note the increasing extent of LDH staining (purple) in middle and lower depths of tissue in burn tissue with time since injury and non-linear pattern of this staining. In control skin, more typical pattern of staining is seen as described in Fig. 1(A). Red dotted line indicates epidermo-dermal junction. Note the absence of epidermal cells in 5/8 of the burn sections. (B) Time since injury and relationship with LDH stain percentage across whole tissue, superficial, middle and deep third burn vs. control skin is presented. Linear regression line of best fit is presented with 95% confidence interval, 95% prediction limits visualised. See supporting information 4, Table 2 for summary table showing slope, proportion of variance explained by the regression line (R²) together and p value, which accompanies Fig. 2B. (C) Box and whisker plot demonstrating average LDH percentage (median, IQR and range) for burn vs. control across whole tissue, superficial third, middle third and deep third of tissue is shown in (D) (n = 38, paired t test). **indicates p value of < 0.05.