Figure 3 | Scientific Reports

Figure 3

From: Lactate dehydrogenase activity staining demonstrates time-dependent immune cell infiltration in human ex-vivo burn-injured skin

Figure 3

(A) Box and whisker plot indicating average number of cells per high powered field (PHF) (40X magnification) between burn and control skin. Cluster of differentiation (CD) 3, 4, 8, 20 and 68 stains. (n = 6). *denotes p value < 0.05 using paired t test. Note a tendency to increased cell density in all CD staining in burn tissue, although not reaching statistical significance in CD8 or CD20 staining. (B) Paired LDH stain (a) from 10 μm fresh frozen section and CD4+ stain (b) and 4 μm formalin fixed paraffin embedded section from adjacent area in same wound. Presented with scale bars. Note bands showing increased formazan deposits in reticular dermis of skin in regions anatomically correlating to regions increased CD4+ positive staining. Red boxes showing highlighted regions to demonstrate correlation between LDH staining and CD4+ staining. Scale bars shown in box.

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