Figure 1
From: Selective and predicable amine conjugation sites by kinetic characterization under excess reagents
The initial rate (v0/NH2) measurement based on the slope (dashed line) of the time course study of the reaction of Her-IgG (5.73 μM) with AD-PEG-N3 (3 mM) (closed) and NHS-PEG-N3 (50 mM) (open). The products (P–L) were monitored as L/P ratios or the converted [P-HL-L] using intact protein measurement (left).
