Figure 3
From: Selective and predicable amine conjugation sites by kinetic characterization under excess reagents
Conjugation levels for Lys sites of Her-IgG (100 μg) (left) generated by reaction with a: NHS-PEG-N3 (7.71 mM) for 1 h, b: AD-PEG-N3 (0.30 mM) for 1 h, c: AD-PEG-N3 (3.0 mM) for 1 h, d: AD-PEG-N3 (2.89 mM) for 4 h with gradual addition, and e: formaldehyde (0.57 mM) for 1 h. The L/P (~ 4) and L/KM ratios are indicated in the bottom of the map. The deconvoluted spectra of the light chain (right top) and heavy chain (right bottom) of “d” and “a” shown in the left.
