Figure 4 | Scientific Reports

Figure 4

From: The regulatory effect of hyaluronan on human mesenchymal stem cells’ fate modulates their interaction with cancer cells in vitro

Figure 4

TGFβ3 increases HA-synthesis in bmMSCs and modulates adipogenic differentiation (a) bmMSCs were treated with TGFβ3 (5 ng/ml) and the amount of secreted HA was analysed with an ELISA-like HABP-binding assay after 48 h. The results were normalised to the protein content of the cell lysates. n = 4. Mean ± SEM. *p < 0.5 compared to control. (b) and (c) BmMSCs were differentiated into adipocytes with or without the addition of 4-MU (300 µM) or TGFβ3 (5 ng/ml) over a period of 28 days. (b) Lipid vesicles of adipogenic differentiated bmMSCs were stained with Oil Red O. Scale bar = 200 µm. (c) Adipogenic differentiation was quantified by determining the area fraction of Oil Red O-stained lipid vesicles and normalised to the number of nuclei. *p < 0.05. n = 4. Mean ± SEM. This figure was prepared with GraphPad Prism (v9.2.0.332, www.graphpad.com).

Back to article page