Figure 5 | Scientific Reports

Figure 5

From: The regulatory effect of hyaluronan on human mesenchymal stem cells’ fate modulates their interaction with cancer cells in vitro

Figure 5

Invasive breast cancer cells interact closely with mesenchymal stem cells. (a) Microscopic pictures of a direct co-culture of bmMSCs (arrows) and the cancer cell lines (asterisk) MDA-MB-231 and U87-MG. The cancer cell lines were labelled with CFSE (green). HA was stained with HABP (red) and the nuclei with Hoechst 33,342 (blue). Scale bar = 50 µm. (b) Percentage of MDA-MB-231 and U87-MG cells co-localising with bmMSCs. (c) The accumulated distance of the tumor cell lines MDA-MB-231 and U87-MG in monoculture was analysed via time-lapse microscopy over a period of 24 h. (d) and (e) Time-lapse analysis of MDA-MB-231 and U87-MG cells cultivated in co-culture with bmMSCs. (d) Accumulated distance and (e) the time spent in juxtaposition of bmMSCs. n = 4. Mean ± SEM. *p < 0.05 compared to MDA-MB-231. This figure was prepared with GraphPad Prism (v9.2.0.332, www.graphpad.com).

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