Table 3 Expression stability values of eight candidate reference genes calculated by BestKeeper.
Rank | Rhizosheath development | Drought | Tissues | All samples |
|---|---|---|---|---|
1 | HIS-3 | GADPH | ARP6 | ARP6 |
R value | 0.803 | 0.984 | 0.973 | 0.944 |
CV ± SD | 2.92 ± 0.73 | 6.43 ± 1.01 | 9.73 ± 2.78 | 6.65 ± 1.76 |
2 | elF | ALDH | elF | PP2A |
R value | 0.777 | 0.968 | 0.958 | 0.937 |
CV ± SD | 2.11 ± 0.46 | 7.55 ± 1.29 | 12.92 ± 3.23 | 9.62 ± 2.00 |
3 | GADPH | elF | PP2A | elF |
R value | 0.655 | 0.924 | 0.958 | 0.933 |
CV ± SD | 4.36 ± 0.71 | 3.86 ± 0.83 | 12.08 ± 3.13 | 8.14 ± 1.86 |
4 | TIP411 | ARP6 | GADPH | ALDH |
Rvalue | 0.48 | 0.918 | 0.918 | 0.931 |
CV ± SD | 2.83 ± 0.63 | 4.05 ± 1.03 | 17.53 ± 3.54 | 11.17 ± 2.00 |
5 | α-TUB | TIP411 | ALDH | GADPH |
R value | 0.325 | 0.914 | 0.907 | 0.929 |
CV ± SD | 13.25 ± 4.25 | 4.06 ± 0.93 | 10.57 ± 2.15 | 12.16 ± 2.12 |
6 | PP2A | α-TUB | TIP411 | TIP411 |
R value | − 0.293 | 0.811 | 0.858 | 0.905 |
CV ± SD | 2.88 ± 0.63 | 2.69 ± 0.92 | 7.86 ± 2.00 | 6.37 ± 1.51 |
7 | ALDH | PP2A | HIS-3 | HIS-3 |
R value | − 0.336 | 0.591 | 0.791 | 0.858 |
CV ± SD | 4.74 ± 0.75 | 3.82 ± 0.83 | 9.48 ± 3.03 | 12.38 ± 3.46 |
8 | ARP6 | HIS-3 | α-TUB | α-TUB |
R value | − 0.734 | − 0.238 | − 0.382 | 0.411 |
CV ± SD | 2.54 ± 0.64 | 5.62 ± 1.48 | 14.11 ± 5.82 | 12.03 ± 4.34 |
