Table 2 Molecular assays used for detection of six drug resistance/compensatory mutations specific of the Beijing 1071-32-cluster.
Gene | Genome position | Method of detection | Primers and RE (in case of PCR–RFLP) | Reference |
|---|---|---|---|---|
KatG 315 AGC/ACC | 2155168 | PCR–RFLP | Fw 5′-GCAGATGGGGCTGATCTACG Rev 5′-AACGGGTCCGGGATGGTG RE: MspI | |
KatG 335 ATC/GTC | 2155109 | PCR–RFLP | Fw 5′-GCAGATGGGGCTGATCTACG Rev 5′-AACGGGTCCGGGATGGTG RE: AvaII | This study |
RpoB 450 TCG/TTG | 761155 | Rifoligotyping | Fw 5′-GTCGCCGCGATCAAGGA Rev 5'-[Biotin]ACGTCGCGGACCTCCA | |
RpoC 485 GAT/AAT | 764822 | Allele-specific PCR | Fw 5′-CTGGAGCTGTTCAAGCCGTTC RevWT 5′-CGATGACCTCTTCGAGCACAGC RevMut 5′-CGATGACCTCTTCGAGCACAGC | This study |
EmbB 497 CAG/CGG | 4248003 | PCR–RFLP | Fw 5′-TGTGCAGCCCACCGGCCTGA Rev 5′-TGTGCAGCCCACCGGCCTGA RE: MspI | This study |
RpsL L43 AAG/AGG | 781687 | PCR–RFLP | Fw 5'-CAGCCCGCAGCGTCGTGGTG Rev 5'-GCTGCGTGCCTGTTTGCGGTTCTT RE: MboII | This study |
