Table 1 RT-qPCR primer sequences in this study.

From: Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells

Gene name

Gene bank number

Sequence(5′–3′)

Tm (℃)

Product size (bp)

ACTB

NM_001101.5

Forward TGGCACCCAGCACAATGAA

69.0

186

Reverse CTAAGTCATAGTCCGCCTAGAAGCA

65.4

BAX

NM_138761.4

Forward CTCAGGATGCGTCCACCAA

67.5

83

Reverse CCTCTGCAGCTCCATGTTACTGTC

68.1

BCL2

NM_000633.2

Forward AACATCGCCCTGTGGATGAC

67.3

146

Reverse AGAGTCTTCAGAGACAGCCAGGAG

66.3

HSPA1A

NM_005345.6

Forward CCTGGAGTCCTACGCTTCAAC

68.0

104

Reverse CTTGACACTTGTCCAGCACCTTC

67.1

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