Figure 4

Effect of Eupafolin on PI3K/AKT, MAPKs and NF-κB signaling in breast cancer cells. (A,B) Eupafolin treatment leads to decreased phosphorylation of PI3K/AKT, Erk1/2, p38, and NF-κB/p65 by western blotting. Full-length images are presented in Supplementary Figs. 5 and 6. Graphs of signal intensity were obtained through band densitometry and referred to GAPDH and control levels. Data is representative of three independent experiments and expressed as mean ± SD. P > 0.05 indicates non-significance; ***P < 0.001; **P < 0.01; *P < 0.05.