Figure 5
From: Lactate activates hypothalamic POMC neurons by intercellular signaling
HCAR1 is absent of POMC neurons. (A) Experimental workflow. (B,C) Size versus cellular complexity plots from FACS purification of POMC-EGFP neurons from transgenic mice. Inserts correspond to FITC intensity distribution of sorted events. (B) POMC-EGFP mice. (C) WT control mouse. (D,E) qRT-PCR of hypothalamus samples and sorted POMC neurons in POMC-GFP mice (N = 3). (F) Expression profiles of Pomc, Agrp, Gfap, Hcar1 and 18 s ribosomal RNA from hypothalamus samples (lane 1), sorted POMC neurons (lane 2) cDNA, RT (−) of sorted POMC neurons (lane 3).
