Figure 3

Hierarchical clustering and classification of the differentially expressed proteins. In panel (a), differentially expressed proteins (DEPs) in the hippocampus are classified into 5 classes, according to their expression pattern in different time points during a seizure cluster. Hippocampal class 1 (HC-class 1) exhibits the lowest expression level at the peak of a seizure cluster and gradually increases the expression toward the end of a cluster and the seizure-free period, and designated as ‘inversely cluster-related DEPs’. Accordingly, HC-class 2 was designated as cluster-onset downregulated DEPs, HC-class 3 as cluster-onset upregulated DEPs, and HC-class 4 as positively cluster-related DEPs. HC-class 5 does not exhibit a specific cluster-related expression pattern. In panel (b), cortex class 2 (CO-class 2) was designated as inversely cluster-related DEPs, CO-class 3 as cluster-onset downregulated DEPs, and CO-class 5 as positively cluster-related DEPs. CO-class1 and CO-class 4 do not exhibit a clinically relevant cluster-related expression pattern.