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Figure 1

From: Functional disruption of cell wall invertase inhibitor by genome editing increases sugar content of tomato fruit without decrease fruit weight

Figure 1

Schematic diagram of CRISPR/Cas9 and Target-AID target sites in SlINVINHs and vector construction. (a) Position of SlINVINH1, SlINVINH2 and three guide RNA targets in SlINVINHs. Boxes indicate exons. The black arrowhead indicates the PKF (proline-lysine-phenylalanine) motif. (b) Schematic map of the CRISPR/Cas9 vector, and (c) Target-AID vector. RB: right border. LB: left border. U6pro: AtU6-26 promoter. gRNA: guide RNA. PcUbi: Petroselinum crispum ubiquitin promoter. Cas9: Streptococcus pyogenes Cas9 gene. nCas9: nickase Cas9 gene. PmCDA1: Petro myzontiformes cytidine deaminase 1 gene. PeathreeAter: 3A terminator. 35S pro: Cauliflower mosaic virus 35S promoter. NPTII: Kanamycin resistance gene. Hspter: heat shock protein gene terminator. (d) Sequences of the three targets. The PAM sequences (NGG) are underlined.

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