Table 2 Methods comparison for calculating sensitivity from empirical test based on Qvarnstrom real-time PCR-positive sample (as gold standard method).
Methods | Sensitivity | Specificity | Accuracy | ||||||
|---|---|---|---|---|---|---|---|---|---|
D.C | Culture | Total | D.C | Culture | Total | D.C | Culture | Total | |
Genotyping PCR (M1) | 52% (11/21) | 62% (8/13) | 54% (13/24) | 91% (10/11) | 95% (18/19) | 85% (7/8) | 66% (21/32) | 81% (26/32) | 63% (20/32) |
Optimal modified genotyping nested PCR (M3) | 95% (20/21) | 100% (13/13) | 96% (23/24) | 64% (7/11) | 95% (18/19) | 50% (4/8) | 84% (27/32) | 97% (31/32) | 84% (27/32) |
Scheikl genotyping nested PCR (M4) | 74% (17/23) | 85% (11/13) | 75% (18/24) | 64% (7/11) | 95% (18/19) | 50% (4/8) | 75% (24/32) | 91% (29/32) | 69% (22/32) |
Genotyping semi-nested PCR (M5) | 95% (20/21) | 100% (13/13) | 96% (23/24) | 73% (8/11) | 95% (18/19) | 63% (5/8) | 88% (28/32) | 97% (31/32) | 88% (28/32) |
