Figure 8
From: Salt inducible kinases 2 and 3 are required for thymic T cell development
Transition to CD4 and CD8 SP cells is reduced in SIK2−/−/SIK3fl/fl/CD4-Cre+/− mice. Thymi were isolated from wild type (WT) and SIK2-/-/SIK3fl/fl/CD4-Cre+/- (KO) mice and analysed by flow cytometry. The number of Thy1.2+ve cells in the thymus along with representative CD4/CD8 plots and numbers of DN, DP and SP cells is shown in (A). Thymocytes were also analysed for expression of cell surface TCRβ. Representative plots of Thy1.2 vs TCRβ in live thymocytes along with quantification of TCRβhigh cells is shown in (B). Analysis of CD4 and CD8 expression in the TCRβhigh cells is shown in (C). Representative histograms for cell surface expression of TCRβ and CD5 in DP, CD4 and CD8 SP cells are shown in (D). Graphs show mean with symbols representing measurements from individual mice. Differences in cell number between wild type and knockout mice were analysed by two tailed Student’s t-test (A, B) or two way ANOVA with Sidak’s post hoc testing. p < 0.05 is indicated by *, < 0.01 by ** and < 0.001 by ***. Graphs show data for 7 mice per genotype.
