Figure 3
From: Expression of truncated Kir6.2 promotes insertion of functionally inverted ATP-sensitive K+ channels

Immunocytochemistry of HEK293 cells transfected with 6xHis-Kir6.2ΔC26. Intact (A, B) and permeabilized (C, D) cells were utilized to explore the orientation of 6xHis-Kir6.2ΔC26 within the cell membrane. Biotinylated anti-6xHis was observed using streptavidin-fluorescein conjugate. Bright-field images (B and D) are provided for reference, and all images are on the same intensity scale.