Figure 4
From: Expression of truncated Kir6.2 promotes insertion of functionally inverted ATP-sensitive K+ channels
Immunocytochemistry of HEK293 cells transfected with 6xHis-EGFP-Kir6.2ΔC26. Intact (A–C) and permeabilized (D–F) cells were utilized to explore the orientation of 6xHis-EGFP-Kir6.2ΔC26 within the cell membrane. EGFP fluorescence (A and D) was observed under all conditions, whereas fluorescence resulting from anti-EGFP-AlexaFluor 594 conjugate was only observed in permeabilized cells (B and E). Bright-field images (C and F) are provided for reference. All fluorescent images are on the same intensity scale.
