Figure 2
From: The rumen microbiome inhibits methane formation through dietary choline supplementation
Dosage response of choline supplementation on CH4 and NH3 production. Choline was supplemented to artificial saliva at 0, 6.5, 13, 26, 39, 52, 100 and 200 mM for 15 days. The scatterplot displays the CH4 production in orange and NH3 concentrations in blue between day 11 and day 15, each dot representing measurement from a single day. The CH4 production was normalized to that of the 0 mM group. The CH4 production was fitted with a 3rd order polynomial regression (y = 6 × 10–07 x3 − 0.0003 x2 + 0.0267 x + 1.1659, R2 = 0.908), the NH3 concentrations was fitted with a 2nd order polynomial regression (y = 0.0078 x2 + 0.5374 x + 9.1634, R2 = 0.982).
