Figure 1 | Scientific Reports

Figure 1

From: p-STAT3 is a PDC-E2 interacting partner in human cholangiocytes and hepatocytes with potential pathobiological implications

Figure 1

PDC-E2 associates with p-STAT3 and STAT3 in unstimulated and GCDC-stimulated cholangiocytes. Normal human cholangiocytes (NHC) stimulated with 100 µM of glycochenodeoksycholic acid (GCDC) were collected and subjected to subcellular fractionation in order to obtain cytoplasmic (Cyto) and nuclear (Nuc) fractions. (A) PDC-E2, pY-STAT3 and STAT3 were detected by using appropriate antibodies and normalized to GAPDH (cytoplasmic fraction) or HDAC-2 (nuclear fraction). Level of each protein was presented as a mean ± SEM. (B and C) Immunoprecipitation (IP) utilized anti-PDC-E2 or anti-STAT3 antibody and contained 500 µg proteins per reaction. PDC-E2 (70 kDa) and pY-STAT3 or STAT3 (both 86 kDa) were detected by Western blot (WB). IP experiments were repeated three times on independent batches of cells.

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