Figure 2 | Scientific Reports

Figure 2

From: p-STAT3 is a PDC-E2 interacting partner in human cholangiocytes and hepatocytes with potential pathobiological implications

Figure 2

PDC-E2 associates with p-STAT3 and STAT3 in GCDC-stimulated hepatocytes. Hepatocytes (Hep-G2) stimulated with 250 µM of glycochenodeoksycholic acid (GCDC) were collected and subjected to subcellular fractionation in order to obtain cytoplasmic (Cyto) and nuclear (Nuc) fractions. (A) PDC-E2, pY-STAT3 and STAT3 were detected by using appropriate antibodies and normalized to GAPDH (cytoplasmic fraction) or HDAC-2 (nuclear fraction). Level of each protein was presented as a mean ± SEM. (B and C) Immunoprecipitation reaction (IP) utilized anti-PDC-E2 or anti-STAT3 antibody and contained 500 µg proteins per reaction. PDC-E2 (70 kDa) and pY-STAT3 or STAT3 (both 86 kDa) were detected by Western blot (WB). IP experiments were repeated three times on independent batches of cells.

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