Figure 3 | Scientific Reports

Figure 3

From: Basal and IL-1β enhanced chondrocyte chemotactic activity on monocytes are co-dependent on both IKKα and IKKβ NF-κB activating kinases

Figure 3

IKK dependency of monocyte recruitment following exposure to chondrocyte conditioned media. (a) Supernatants obtained from high density cultures (n = 7) of GL2 (black pattern), IKKαKD (gray pattern) or IKKβKD (white pattern) chondrocytes in control condition (−) or after IL-1β stimulation (24 h, +) were collected and assessed for the level of monocyte chemotaxis in Boyden Chambers. Data are reported as mean ± SD. The means of the groups were compared by ANOVA, followed by Tukey’s post hoc test. The differences were considered significant when p < 0.05 with *p < 0.05; **p < 0.01; and ***p < 0.001. (b) The extent of chemotaxis in term of number of migrated cells is highly correlated to CCL2/MCP-1 concentration (pg/ml), as evaluated by mean of the Pearson r (r = 0.428, p = 0.02, two-tailed, n = 30). Different symbols refers to basal (full black circles) or IL-1β stimulated samples (full gray boxes). (c) MCP-1 is expressed in osteoarthritic cartilage as assessed by immunohistochemistry (on the left isotype control; on the right cartilage section probed with anti-MCP-1 antibody, ×4 magnification and ×40 insert).

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