Figure 4 | Scientific Reports

Figure 4

From: Basal and IL-1β enhanced chondrocyte chemotactic activity on monocytes are co-dependent on both IKKα and IKKβ NF-κB activating kinases

Figure 4

IKK dependency of p65phosphorylation. (a) A representative western blot (out of four performed) showing the kinetic of p65 phosphorylation (p65pSer536) in high density cultures of chondrocytes with either the control shRNA (GL2) or with IKKαKD or IKKβ KD. The cultures were either unstimulated (0) or treated with 2 ng/ml IL-1β for 30, 60 or 120 min. At the end of stimulation, the cells were recovered by trypsinization and counted. Total proteins derived from equal cell equivalents were run and blotted. β actin was used as a loading control. (b) Data obtained from the four experiments underwent densitometric analysis, and the cumulative results reported as mean ± SD fold increase the level of the control unstimulated GL2. The means of the groups were compared by the Student’s t test. The differences were considered significant when p < 0.05 with *p < 0.05. Different patterns are used for different phenotypes: GL2 black, IKKαKD gray, IKKβKD white. Both the basal and the 60 min p65pSer536 levels were higher in GL2 compared the IKKαKD level.

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