Figure 1
From: Osteocrin ameliorates adriamycin nephropathy via p38 mitogen-activated protein kinase inhibition
Urinary albumin levels were lower in OSTN-Tg mice than in control mice under ADR nephropathy. (a) Expression of Ostn mRNA in various tissues of wild-type mice. Skin (skin of the auricular region). (b) Schematic of the experimental protocol. OSTN-Tg or control mice were injected intravenously with saline or ADR (8 mg/kg) at 6 weeks of age, and sacrificed at 10 weeks of age. (c) Immunohistochemical study for NPR-C. Bars, 20 μm. (d–g) Immunofluorescent studies for NPR-C (d; red), nephrin (e; green), and merged images (f,g) in four groups. (d–f) Bars 20 μm, (g) Bars 4 μm. (h) The BW at weeks 5, 6, 7, 8, 9 and 10 and right kidney weight, serum creatinine levels at weeks 10 (n = 7–8 per group). *P < 0.05, CT + vehicle vs. Tg + vehicle, or CT + ADR vs. OSTN KO + ADR. **P < 0.01, ***P < 0.001 by one-way ANOVA analysis. (i) Urinary albumin creatinine ratio (UACR) at weeks 5, 7, 8, 9 and 10 (n = 7–8 per group). Tg 61.9 ± 8.5 μg/mgCr vs. CT 91.5 ± 33.7 μg/mgCr. BW, body weight; CT, control mice; Tg, OSTN-Tg mice. Data are mean ± SD. *P < 0.05 by one-way ANOVA analysis.
