Figure 2
From: Osteocrin ameliorates adriamycin nephropathy via p38 mitogen-activated protein kinase inhibition
ADR-induced podocyte injury was ameliorated in OSTN-Tg mice. (a) Light microscopic analysis stained with PAS. Bars, 20 μm. (b) Immunohistochemical study for desmin (magnification, × 400). Bars, 20 μm. (c,d) Electron microscopic analysis with lower magnification (c; magnification, × 2000, Bars, 10 μm), and higher magnification (d; magnification, × 15,000, Bars, 500 nm). (e) Desmin-positive areas were analyzed. ADR Tg 0.42 ± 0.35 μm2 vs. ADR CT 2.02 ± 0.63 μm2. (f) Thickness of glomerular basement membrane (GBM) and width of foot process effacements were measured. GBM thickness: Tg 140.9 ± 13.2 nm vs. CT 208.7 ± 43.1 nm, P < 0.001; width of foot process: Tg 268.3 ± 19.3 nm vs. CT 502.7 ± 42.6 nm. PAS periodic acid-Schiff staining, EM electron microscopy, CT control mice, Tg OSTN-Tg mice. Data are mean ± SD. ***P < 0.001 by one-way ANOVA analysis.
