Figure 3
From: Osteocrin ameliorates adriamycin nephropathy via p38 mitogen-activated protein kinase inhibition
ADR-induced inflammation was ameliorated in OSTN-Tg mice. (a) Glomerular p38 MAPK phosphorylation in four groups at 10 weeks of age. The grouping of gels cropped from the same lines of the same gel. Full-length blots/gels are presented in Supplementary Fig. S13a. (b) Glomerular mRNA expression levels of Ccl2 in four groups at 10 weeks of age. (c–e) Immunofluorescent studies for MCP1 (c; red), nephrin (d; green), and merged images (e) in four groups. (f,g) Immunohistochemical findings of MAC-2 (f), a macrophage marker, and the number of MAC-2-positive cells in glomeruli in four groups (g). phospho p38, phosphorylated p38 MAPK; CT, control mice; Tg, OSTN-Tg mice. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA analysis.
