Figure 3

An example of obtaining MLLSV. The detection threshold of HAP (uricacid) map is set as − 100 ~ 100 mg/cm3. The lower limit of detection threshold (mg/cm3) is adjusted on the uricacid (HAP) map. Multiple stained sites [red arrow in (A)] were shown in the left first MTP joint with lower limit of 1310 mg/cm3 (red rectangle) of the uricacid (HAP) map [black arrow in (A)]. The number of stained sites [arrow in (B)] decreased at lower limit of 1330 mg/cm3 [red rectangle in (B)]. Only one stained site [arrow in (C)] is visible with the lower limit of 1350 mg/cm3 [red rectangle in (C)]. The last stained site is not seen with lower limit of 1351 mg/cm3 [red rectangle in (D)]. MLLSV is thus 1350 mg/cm3 for this case. MLLSV = maximum lower limit with stain visible. HAP (uricacid) = hydroxyapatite (uric acid), uricacid (HAP) = uric acid (hydroxyapatite).