Figure 1
From: miRNA-200c-3p targets talin-1 to regulate integrin-mediated cell adhesion
Regulation of miR-200c-3p, ZEB1, and TLN1 expression in HEK293T cells. HEK293T cells were stably transduced with miR-200c-3p pmiRZip to down-regulate miR-200c-3p expression (A–C), miR-200c-3p pre-miR (miR-200c-3p mimic) to upregulate miR-200c-3p expression (D–F), or an empty vector (control), using lentivector systems. The expression levels of miR-200c-3p (A, D), ZEB1 (B, E), and TLN1 (C, F) in three representative clones per group were measured using RT-qPCR. The control values in each panel were normalized to 1. All assays were performed in triplicates and the experiments were repeated three times. Data are expressed as the mean ± standard errors of the mean (SEM) relative to the control. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.
