Figure 3
From: miRNA-200c-3p targets talin-1 to regulate integrin-mediated cell adhesion
miR-200c-3p targets the 3′-UTR of TLN1. (A) Schematic representation of the two conserved binding sites for miRNA-200c-3p on TLN1 3′-UTR and their potential interactions with miRNA-200c-3p seed sequences. The miR-200c-3p seed sequences and their target sequences are underlined. (B) Luciferase reporter activities were measured, 48 h after HEK293T cells were co-transfected with the TLN1-3′-UTR-luciferase construct and miR-200c-3p pmiRZip, miR-200c-3p mimic, or control vector (Cont. vec.). All assays were performed in triplicates and experiments were repeated three times. Data are expressed as the mean ± standard errors of the mean (SEM) relative to the control vector. **P < 0.01; and ****P < 0.0001.
