Figure 2

Expression pattern and temporal traits of RfVg gene transcription. (A) Expression pattern of RfVg and tubulin genes from different tissues of Rhynchophorus ferrugineus was analyzed by RT-PCR. Agarose gels (2%) were used to analyze the amplified PCR products. The M is a molecular marker (bp), while 640 bp and 196 bp on right side are amplified products of Vg and tubulin genes, respectively. (B) The RfVg and tubulin genes expression profile up to three weeks in the adult R. ferrugineus females analyzed by RT-PCR. The amplified bands were visualized under the UV light and photographed by using gel documentation BioDocAnalyze system (Biometra). d days.