Figure 3

The RNAi-based silencing of RfVg gene results in significant down regulation of Vg gene. Expression levels of the RfVg gene were quantified by quantitative real time PCR. Normalized fold expression of the RfVg-dsRNA-injected group were compared with no injection (NI) and nucleus free water (NFW) groups on day 15, 20, and 25 of post-injection periods. Different letters above the bars (a,b) show significant differences among the groups at (α = 0.05). The expression levels of RfVg gene were significantly reduced in RfVg-dsRNA injected group in comparison to the NI injection and NFW injected groups. All biological groups contained three replicates. Each replicate has a single animal, while there were three technical replicates.