Figure 4 | Scientific Reports

Figure 4

From: Silencing of vitellogenin gene contributes to the promise of controlling red palm weevil, Rhynchophorus ferrugineus (Olivier)

Figure 4

Validation of RNAi and expression analysis of RfVgs by SDS-PAGE. To clarify the RfVg protein expression, hemolymph samples (10 μl/lane) prepared from NI, NFW, and RfVg-dsRNA injected groups on 15, 20, and 25 days (d) post-injection periods were loaded on SDS-PAGE (8%) and compared. All biological groups contained three replicates; each replicate was an individual R. ferrugineus, while there were three technical replicates. The gel was stained with Coomassie blue and washed with destaining solution. M is the molecular marker (kDa) while, arrows on right side indicate the identified Vg polypeptides (175 and 45 kDa) in R. ferrugineus. A highly downregulated expression of Vg protein in RfVg-dsRNA injected group as compared to NI and NFW injected groups has been shown with the red circles. The protein bands were visualized under the white light and photographed by using gel documentation BioDocAnalyze system (Biometra). d  days.

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