Figure 6

Phenotypic effects of RfVg-based RNAi on ovarian/egg development of Rhynchophorus ferrugineus. (A) To observe the impact of RNAi on ovarian development, ovaries from three biological groups (RfVg-dsRNA, NFW, and NI) were dissected 20 days post injection period, observed under a stereomicroscope (DM 165 C, Leica, Wetzlar, Germany) and compared by using auto-montage software system (Syncroscopy, Cambridge, UK). (B) The eggs laid by NI, NFW, and RfVg-dsRNA-injected females were observed under the stereomicroscope (Leica MZ 125, Germany), and compared by using the auto-montage software (Helicon focus 6). Scale bars: 10 mm and 1.0 mm for ovaries and eggs, respectively.