Figure 3
Tissue specific expression analysis of SlBAGs performed using qRT-PCR. Transcript accumulation of SlBAGs was measured by qRT-PCR using tomato actin as endogenous control. qRT-PCR data was used to make a heat map for displaying gene expression using clustvis tool (https://biit.cs.ut.ee/clustvis/)48. (A) Heat map showing SlBAGs expression during different plant tissues (B) Heat map showing SlBAGs expression during fruit developmental and ripening stages. After tagging flowers at anthesis, fruits were harvested at 7 to 20 days after anthesis (DAA), mature green, breaker, pink and red ripe stages of fruit development and ripening. Each qRT-PCR reaction was performed with three independent biological replicates, each consisting of three independent technical replicates. The 2−ΔΔCT method for analyzing the qRT-PCR data was employed. The values were converted to log2 fold change in expression before making the heat map.
