Figure 4

qRT-PCR based expression profiling of SlBAGs during abiotic stress conditions. Transcript accumulation in 15-day-old tomato seedlings exposed to 200 mM NaCl for salt stress, 250 mM mannitol for osmotic stress, heat and cold was measured by qRT-PCR using tomato actin as endogenous control by considering reference expression level 1 at 0 h time point using 2^−ΔΔCT method. All data are presented as the mean (± SE) of three independent biological determinations and analysed using Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001).