Figure 5
qRT-PCR based expression profiling of SlBAGs after hormonal treatment to tomato seedlings. Transcript accumulation in 15-d-old tomato seedlings exposed to 1 mM ACC, 0.1 mM ABA, 0.02 mM IAA, 0.02 mM GA3 and 0.02 mM zeatin was measured by qRT-PCR using tomato actin as endogenous control by considering reference expression level 1 at 0 h time point using 2−ΔΔCT method. All data are presented as the mean (± SE) of three independent biological determinations and analysed using Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001).
