Figure 11
From: Functional roles of the membrane-associated AAV protein MAAP
Effect of MAAP inactivation on kanamycin resistance gene packaging. wt-AAV2 or AAV2 MAAP variants and Ad helper plasmids were co-transfected in 293 T cells. At 24 hpt (A) and 72 hpt (B), AAV vg titers were quantified. In parallel, the kanamycin resistance gene carried by the AAV2 genome and the Ad helper plasmids was quantified. We present the ratio of kanamycin resistance gene packaging relative to the AAV2 genome packaging, expressed as percentage. Individual samples are represented, along with the mean and the standard deviation. Statistical significance between the wt-AAV2 and AAV encoding MAAP mutants was evaluated using ANOVA followed by Dunnett’s multiple comparison test. Tables (C) and (D) show the average percentage of kanamycin resistance gene packaging relative to AAV2 genome packaging, measured for each virus at 24 hpt and 72 hpt, and the fold difference compared to wt-AAV2. Experiments were performed independently between 3 and 6 times (indicated as N), each time with two samples.
