Figure 2
From: Functional roles of the membrane-associated AAV protein MAAP
MAAP start codon identification and MAAP inactivation. (A) MAAP detected by immunoblot from 293 T cell lysates 24 hpt with wt- or MAAP mutants and Ad helper plasmids. Immunoblots of MAAP (top panel) and of α-Tubulin (lower panel) for equal sample loading. Left to right: (1) MW. (2) wt-AAV2. (3) AAV2 with MAAP-L1 (CTG) start codon modified to R1 (CGG). (4) MAAP-Q9. (5) MAAP-S39. (6) MAAP-S33-S39-S47. (7) non-transfected 293 T cells. (B) Start codon usage for MAAP translation. MAAP expressed from the first or second potential start codon and detected by immunoblot. Left to right: (1) MW. (2) wt-AAV2. (3) MAAP expressed from L1 (CTG) mutated to M (ATG). (4) MAAP expressed from R13 (AGG) mutated to M (ATG). (5) wt-AAV2. (6) v/v mix of MAAP expressed from L1 (CTG) mutated to M (ATG) and MAAP expressed from R13 (AGG) mutated to M (ATG). (7) MW. (8) non-transfected 293 T cells.
