Figure 3
From: Improved production of the non-native cofactor F420 in Escherichia coli
Flux balance analysis of TCA cycle and anaplerotic reactions of the TCA cycle (A) along with glycolysis/gluconeogenesis (B) pathways predicted by iEco-F420 metabolic model of E. coli for independent simulations using glucose, glycerol, or succinate as sole carbon sources (60 C-mol of carbon source). Objective is maximizing F420 production while maintaining growth at 30% of its max. Maintenance ATP requirements is fixed at 5.17 mmol/g DCW. Colormap shows absolute flux values in mmol/gDCW/hr. Fructose-bisphosphate aldolase, Triose-phosphate isomerase, Glyceraldehyde-3-phosphate dehydrogenase, and phosphoenolpyruvate hydratase are active in favor of gluconeogenesis pathway with succinate as the carbon source.
