Figure 1 | Scientific Reports

Figure 1

From: A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein

Figure 1

Peptide Microarray to Identify SARS-CoV-2 S Protein Binding Sequences. (a) Schematic showing microarray screening procedure for detecting binding of a biotinylated target protein. (b) Microarray images of peptide subarray following exposure to 50, 10, or 5 µg/mL of SARS-CoV2 S1 protein. (c) Normalized binding signal of peptides after exposure to SARS-CoV2 S1 protein at 50, 10, or 5 µg/mL concentration. Z-score at each S1 concentration is plotted for peptides listed in numerical order along the X-axis. P2-P811 correspond to peptides derived from the ACE2 N-terminal alpha helix (approach 1) while P812-P2376 were derived from a random starting library and screened in silico for docking to S protein (approach 2). Peptides with high Z-scores (> 1.95, indicated by dotted line) represent S1 protein binding sequences.

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