Figure 3
Binding characterization of peptide binders. (a–d) BLI binding traces of S1 protein association and dissociation to each of the top four biotinylated peptides (immobilized onto streptavidin sensors). (e) The calculated binding constants for the top four peptides. (f) Potential binding sites for the top-4 peptides on the RBD subunit of the spike protein (colored in cyan) as identified from the docking simulations. Peptides P89 (red) and P100 (blue) bind to the ACE2 binding site, peptide P180 (yellow) binds to the same site as CR3022 and S2A4 antibodies, while the binding site for peptide P168 (purple) is located similarly to the antibody S309 binding site25,30,31,32.
