Figure 1
Male mice lacking one allele of Mdm2 in their adipose exhibit accentuated fat accumulation and impaired insulin sensitivity when fed a high-fat diet. (a) Weight gain of male and female wildtype (N = 8 and 10, respectively) and Mdm2Adi+/− mice (N = 7 and 9, respectively). Mice were 6 weeks of age at the onset of high-fat feeding. (b) Fat and lean mass of wildtype (N = 8) and Mdm2Adi+/− (N = 7) male mice scored by MR scanning after 10 weeks of high-fat feeding. (c) Weight of isolated tissues after 15 weeks on high-fat diet. (d) Histological inspection (top) and macrophage marker gene expression (bottom) by real-time qPCR of epiWAT, ingWAT and intBAT from high-fat fed wildtype and Mdm2Adi+/− mice after 15 weeks on high-fat diet. Tnfa, Tumor necrosis factor alpha; Cd68, Cluster of differentiation 68; Adgre1, Adhesion G protein-coupled receptor E1. (e) Insulin tolerance test after 13 weeks on high-fat diet. (f) Oral glucose tolerance test after 11 weeks on high-fat diet. (g, h) Real-time qPCR-based quantification of Adn, Fabp4, Glut4, Atgl and Lipe in (g) epiWAT, ingWAT and intBAT of high-fat fed wildtype and Mdm2Adi+/− mice after 15 weeks on high-fat diet or in (h) 3T3-L1 adipocytes with knockdown of Mdm2. Adn, Adipsin; Fabp4, Fatty acid binding protein 4; Glut4, glucose transporter 4; Atgl, adipose triglyceride lipase; Lipe, hormone sentitive lipase. (h) Insert, western blot analysis of MDM2 and α-Tubulin. For a, e, and f, significance was tested using two-way ANOVA with Bonferroni-correction, * = p-value < 0.05. For b, c, d, g, and h, significance was tested using Student’s t-test, * = p-value < 0.05.
