Figure 2
High-fat fed Mdm2Adi+/− mice suffer from hepatic steatosis. (a, c, d, g, h) Male wildtype (N = 8) and Mdm2Adi+/− (N = 7) mice after 15 weeks on high-fat diet. (a) Relative triglyceride content in livers normalized to weight. Triglycerides were extracted using isopropanol and quantified with an enzyme-based colorimetric assay. (b) Histological analysis of H&E-stained sections of livers. (c) mRNA levels of genes encoding lipid-handling proteins measured by real-time qPCR. Cd36, Cluster of differentiation 36; Scd, Stearoyl-CoA desaturase; Fabp1, Fatty acid-binding protein 1; Me1, malic enzyme 1. (d) Quantitative proteomic comparison of epiWAT from wildtype and Mdm2Adi+/− mice fed a high fat diet for 15 weeks. Volcano plots of quantified proteins generated in Perseus70. FDR was set to either 0.05 or 0.01. s0 held at 0.1. (e) Enriched biological processes amongst the significantly up- or down-regulated proteins using DAVID 6.871,72. (f) MS1 spectrum of a MUP1 (Major Urinary Protein 1) peptide (DGETFQLMGLYGREPDLSSDIK) from wildtype and Mdm2Adi+/− mice. Depicted using Xcalibur. (g) ELISA-based quantification of serum levels of MUP1 in wildtype and Mdm2Adi+/− mice after 15 weeks on high-fat diet. (h) mRNA levels of Mup1 in adipose depots, liver, and muscle of high-fat fed wildtype and Mdm2Adi+/− mice as scored by real-time qPCR. For a, c, g and h, significance was tested using Student’s t-test, * = p-value < 0.05. For d, p-value was calculated using Student’s t-test. For e, p-value was calculated using Fisher’s Exact test with Benjamini correction.
